Composite

Part:BBa_K575045

Designed by: Kristin Palarz   Group: iGEM11_Northwestern   (2011-09-22)

Genomic LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP, Cons. Promoter + RBS (B0030) + LasR

This construct contains a LasR/PaI1 (3-oxo-C12-HSL) inducible promoter from the Pseudomonas genome with an RBS and a GFP reporter. There is also constitutive transcription of the LasR receptor protein. Thus, the promoter will activate and fluorescence will be observed in the presence of PAI1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1281
    Illegal NheI site found at 1304
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1660
    Illegal AgeI site found at 1857
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1190


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Categories
Parameters
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